Optimization of PCR conditions for Agriophyllum, Haloxylon and Salsola microsatellite markers
نویسندگان
چکیده
منابع مشابه
Microsatellite genotyping of post-PCR fluorescently labeled markers.
We show that a post-PCR multicolor fluorescence-labeling technique is applicable to multiplex microsatellite genotyping. Forty-three dinucleotide microsatellite markers, which are located on 11q13-23, a candidate region for dominant familial exudative vitreoretinopathy (FEVR), were used to evaluate the quality of the marker profile produced by this technique. Thirty-eight people from six famili...
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The authors would like to thank Sonja Scheffer for help with DNA sequencing and Roberta Henegar, Sharon Ochs, Nicole Guimond, and Patrice Armstrong for excellent technical assistance. Mention of a trade name or commercial product in this publication is solely for the purpose of providing specific information and does not imply recommendation or endorsement by the U.S. Department of Agriculture....
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An important everyday task for geneticists and molecular biologists is that of isolating and analyzing some particular DNA regions (markers), each drawn from a limited and known set of possible values (alleles). This procedure is called genotyp-ing and is based on DNA ampliication and size separation. In order to increase the throughput of genotyping, recently a new experiment has been proposed...
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ژورنال
عنوان ژورنال: International Journal of Biology and Chemistry
سال: 2020
ISSN: 2218-7979,2409-370X
DOI: 10.26577/ijbch.2020.v13.i1.06